Composite

Part:BBa_K1021016:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)


PT7+crtE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 776
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part does not contain a bacterial RBS and is therefore suited for eukaryotic expression.


Source

The crtE gene comes from Pantoea ananatis and the promoter originates from the T7 virus.

References